G2PzolTM is a cost-effective, single-step RNA isolation reagent that serves as a suitable alternative to TrizolTM , a commonly used RNA isolation reagent. This reagent is capable of effectively purifying RNA from a wide range of sample types, including animal and plant tissues, blood, bacteria, and other sources. The key advantage of G2PzolTM is its ability to deliver high-quality RNA with minimal/NO DNA contamination.
This is accomplished through the use of a specially designed lysis buffer and proprietary purification methods, which result in the efficient partitioning of genomic DNA into the organic phase and protein into the intermediate layer while the RNA partitions into the aqueous layer on top. The DNA-free RNA purified using G2PzolTM is suitable for downstream applications that are sensitive to the presence of residual DNA, such as quantitative PCR (qPCR) and reverse transcription PCR (RT-PCR). This makes G2PzolTM an invaluable choice for researchers seeking to minimize the impact of unwanted contaminants on their results.
A single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNA can then be isolated from the aqueous supernatant by alcohol/isopropanol precipitation. The resulting RNA is intact showing distinct bands of 28S (23S), 18S (16S) and 5.5S (5S) rRNA with little or no contaminating DNA and protein.
Figure1: Total RNA isolation from Mouse brain
Lanes: 1-7 : 5 ul of Total RNA extracted from diff mice brain (Note 3 clear bands of 28S, 18S and 5.5S rRNA)
Lane 8: 1 kb ladder