GenScript’s custom recombinant protein provides a comprehensive portfolio of recombinant protein expression systems that include :
Bacterial Protein Expression system
BacPower Guaranteed Bacterial Recombinant Protein Expression Service
Deliverables:
- Protein in buffered solution with your specified amount & purity – Guaranteed
- Optimized gene sequence report
- QC data– Purity analyzed & detection using SDS-PAGE & WB, Bradford assay for quantitation & Protein delivery
Mammalian Expression system
Transient Expression Service
Deliverables:
- Synthetic gene (as optional for semi-customizable)
- Target protein
- QC data -SDS-PAGE and/or HPLC will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration
Stable Cell Line Development Services
Stable Cell Line Development Services
- Host cells: CHO, CHO-K1, Glutamine Synthetase-knockout CHO-K1, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, U-2OS, or customer-specified cell line.
- Genes: Antibodies, GPCRs, ion channels, proteases, kinases, transmembrane proteins and more.
Baculovirus Expression system
BacuVance Baculovirus Expression Service
Deliverables:
- Construct report
- 5 ml, >108 pfu/ml virus stock
- Expression data
- Purified proteins
- *QC data- SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and Western blot using anti-His or anti-GST antibodies will be performed, to determine protein identity. LC-MS/MS peptide mapping will be used in addition to Western Blot, to determine protein identity (if necessary)
Other Protein Expression systems
PROTential Protein expression service
PROTential Protein expression evaluation & optimization service is to test expression conditions of client gene(s) in the expression system(s) of their choice. It is a small scale Expression Evaluation available in customized packages with below features.
- Evaluate whether the target protein expresses in chosen expression system
- Identify the construct & conditions that gives you the most robust soluble expression of your target protein
- Identify the best expression system for your target protein
Final Deliverables:
Only the expression report is delivered. The protein is consumed in the evaluation experiment and hence does not form part of the deliverables.
Protein sequences are subject to pre-project analysis. Depending upon the nature of the protein and its sequence, different expression hosts and conditions may be suggested. For membrane proteins, toxic proteins and large proteins (>800 aa), special modifications may be suggested.
