1 Kb DNA Ladder

Description:

Gene to Protein Pvt Ltd provides a unique combination of PCR products and proprietary plasmids that are digested using specific restriction enzymes, resulting in 13 fragments. These fragments serve as ideal molecular weight standards for agarose gel electrophoresis, covering a range of 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The estimated DNA mass for each band (0.5 μg per load) aids in approximating the mass of DNA in other similarly sized samples with comparable intensity.

Advantages:

1. Size determination: The 1 kb DNA ladder helps determine the size of double-stranded DNA fragments ranging from 100 bp to 1500 bp. It enables researchers to estimate the size of unknown DNA molecules based on their mobility in an electrical field through the gel.
2. Quantification and comparison: By determining the size of DNA fragments, researchers can quantify DNA fragments and compare their relative sizes. This information is valuable for various applications, including distinguishing between DNA variants and quantifying the number of mutations in a DNA fragment.
3. Molecular detection: The 1 kb DNA ladder can be used for molecular detection of pathogens and characterization of genetic variability. It aids in the molecular tagging of disease resistance genes.
4. Visualization: The 1 kb DNA ladder stains well with nuclear stains like ethidium bromide, allowing researchers to visualize DNA fragments after gel electrophoresis.