Sal I

Description:

SalI is a type II restriction enzyme that recognizes the double-stranded DNA sequence G^TCGAC and cleaves after the G-1

Applications:

1. Molecular cloning: Sal I is commonly used in molecular cloning techniques to insert genes into plasmids.
2. Restriction site mapping: Sal I can be used to map restriction sites on DNA molecules.
3. Genotyping: Sal I can be used for genotyping studies to identify genetic variations.
4. Southern blotting: Sal I can be used in Southern blotting techniques to detect specific DNA sequences.
5. Restriction fragment length polymorphism (RFLP): Sal I can be used in RFLP analysis to study genetic variations.
6. SNP analysis: Sal I can be used to analyze single nucleotide polymorphisms (SNPs).