Cy3-azide is a reactive dye containing an azide group that can participate in click-chemistry reactions. It is used to detect or label molecules with alkyne- or cyclooctyne-containing moieties by fluorescence spectroscopy following azide-alkyne cycloaddition.
Applications:
Protein labeling: Cy3-azide can be used to label proteins with alkyne- or cyclooctyne-containing moieties for detection by fluorescence spectroscopy.
Cell imaging: Cy3-azide can be used to label cells for fluorescence microscopy and flow cytometry.
Biomolecule labeling: Cy3-azide can be used to label biomolecules such as DNA, RNA, and peptides for detection by fluorescence spectroscopy.