PCR (Polymerase Chain Reaction): PCR is a widely used technique that amplifies specific regions of DNA. Blood DNA can be used as a template for PCR to study genetic variations, diagnose genetic diseases, or detect infectious agents.
Restriction Enzyme Digestion: Blood DNA can be digested with restriction enzymes to prepare it for subsequent manipulation. This process involves using between 0.5 and 10 μg of DNA, and the resulting fragments can be analyzed or used for further experiments.
Cloning: Cloning involves digesting blood DNA with restriction enzymes and ligating the fragments into a plasmid or other vector. This technique is used to create libraries, study repeats and expansions in genomic DNA, and perform site-directed mutagenesis.
Genotyping: Blood DNA can be used for genotyping applications, such as identifying genetic variations or single nucleotide polymorphisms (SNPs). This information can be used in genetic research, personalized medicine, or forensic analysis.
Gene Expression Analysis: Blood DNA can be used to study gene expression patterns in different tissues or under different conditions. Techniques such as microarrays or RNA sequencing can provide insights into gene regulation and cellular processes.
Next-Generation Sequencing (NGS): NGS technologies allow for high-throughput sequencing of DNA samples, including blood DNA. This enables whole-genome sequencing, targeted sequencing, or transcriptome analysis.
Paternity testing
Result
It should have an A260/A280 ratio of ∼1.8. Typical DNA yields should be ∼3-5 ug from a healthy human blood sample.
Fig: Blood DNA isolated using Whole blood DNA isolation kit. Lane 1-4, whole blood DNA isolated from 4 different samples. Lane 6, 1 Kb Ladder.
