Description:
NcoI is a restriction endonuclease that is used in molecular biology applications to cleave the DNA recognition site 5′-C/CATGG-3′, resulting in DNA fragments with 5′-cohesive termini. It is derived from an E. coli strain with a cloned NcoI gene from Neisseria corrodens.
Applications:
- Molecular cloning: NcoI is commonly used in molecular cloning techniques to insert genes into plasmids.
- Restriction site mapping: NcoI can be used to map restriction sites on DNA molecules.
- Genotyping: NcoI can be used for genotyping studies to identify genetic variations.
- Southern blotting: NcoI can be used in Southern blotting techniques to detect specific DNA sequences.
- Restriction fragment length polymorphism (RFLP): NcoI can be used in RFLP analysis to study genetic variations.
- SNP analysis: NcoI can be used to analyze single nucleotide polymorphisms (SNPs).
